Hitrap capto sp impres cytiva, 17546851, pack of 5. Capto sp impres is a sulfonate group while the ligand of capto q impres is a quaternary amine group fig 2. Resin fulfills industrial demands for security of supply, robust performance, and regulatory support designed for large feed volumes capto sp impres is a strong cation exchanger for the highthroughput intermediate purification and polishing steps of a wide range of biomolecules. The capto product range comprises modern media that meet the. Capto adhere impres multimodal chromatography resin cytiva. Before packing, transfer the resin to the packing solution as described in the packing instructions for the relevant column. Hitrap capto sp impres is a strong cation exchanger prepacked with bioprocess capto impres media for screening and smallscale protein purifications using ion exchange chromatography iex. Instructions28980216ac prepackedcolumns hiscreencapto. Filled with stationary phase compositions or chemical compounds, the prepacked options reduce the. Capto sp impres and capto mmc impres are supplied in 20% ethanol with 0. Capto phenyl impres is a hic resin for highresolution intermediate and polishing steps in a downstream purification process. The pressureflow specifications for capto s, capto q, and capto deae in large columns is 700 cmh, with less than 3. Packing capto adhere and capto mmc using verified methods. A shorter residence time higher flow velocity of 2 min could be used with capto adhere impres, although this resulted in slightly poorer clearance of aggregates.
Enter a search term to refine what youre looking for. Capto phenyl impres hydrophobic interaction chromatography. Capto s, capto q and capto deae are based on the same high flow agarose matrix, which is designed to give low back pressures at high flow velocities. Data file 11002576 ag ion exchange chromatography capto s, capto q, and capto deae fig 1. Capto sp impres ion exchange chromatography resin cytiva. Capto sp impres cytiva, 17546810, pack of 25 ml sigma. Capto mmc impres screening for binding and elution conditions fig 1. Capto phenyl impres and capto butyl impres are hydrophobic interaction chromatography hic media resins developed for the intermediate and polishing steps in. Gehealthcare lifesciences instructions28980216ac prepackedcolumns hiscreencaptospimpres hiscreencaptoqimpres.
Packing capto impres resins with verified packing methods cytiva. Type of purification manual or system purification system purification format bottles of chromatography resins smallcolumn cartridges. Data file 29067018 ab ion exchange chromatography capto s. Selection guide ion exchange chromatography columns and.
Flow rate capto q impres is a strong anion exchanger prepacked with bioprocess capto impres media for screening and smallscale protein purifications using ion exchange chromatography iex. Capto q impres and capto adhere impres are supplied in 20% ethanol. Both chromatography media resins are part of platform of. Capto sp impres is a strong cation exchanger for the highthroughput intermediate purification and polishing steps of a wide. Polishing of monoclonal antibodies using a polymer grafted. Packing capto impres resins with verified packing methods. Ion exchange chromatography columns for protein purification.
Filled with stationary phase compositions or chemical compounds, the prepacked options reduce the preparing time. Capto sp impres capto s impact capto s capto q xp predictor capto polishing screening plates capto iex selection kit predictor capto polishing screening plates mab. Storage solution predictor robocolumn capto s impact, capto s, capto sp impres, capto mmc impres, sp sepharose ff, sp sepharose hp. Capto phenyl impres and capto butyl impres are hydrophobic interaction chromatography hic media resins developed for the intermediate and polishing steps in a downstream protein purification process fig 1.
Platform process alternatives for mabs based on two or three chromatographic steps. Capto q and capto s are strong ion exchange media for packed bed chromatography that increase speed and. Procedure 29108295 aa multimodal chromatography media. The chromatograms show that aggregates elute at the tail for the peak for both media green traces. Capto q impres ion exchange chromatography resin cytiva. Hitrap columns, capto sp impres, cytiva formerly ge. Before packing, transfer the resin to the packing solution as described in the packing instructions. Capto adhere impres multimodal chromatography resin this multimodal, strong anion exchange resin supports effective mab polishing in the second or third step of a purification scheme downstream of the protein a capture step. A common threestep process begins with a protein a capture step, followed by polishing steps that employ traditional iex techniques e. Typical packing factors pf for capto adhere and capto mmc in different solutions in bpg columns. Product support with a high binding capacity and small particle size, capto s impact is a good choice for reliable and robust polishing steps in an industrial purification process. Application capto sp impres has a strong sulfonate cation exchanger coupled to a chemically modified, highflow agarose matrix. Data file 28930581 ai process development hiscreen.
Gehealthcare lifesciences instructions28977655ae ionexchangemedia captospimpresandcaptoq impres captospimpresandcaptoqimpresarestrongcationandstronganion. Approximately 5 ml of q or sp sepharose fast flow were equilibrated in 1 m kcl and titrated with 100 mm naoh. Available in several formats, including predictor 96well filter plates and prepacked hitrap and hiscreen columns. Jul 02, 2018 iex columns recommended by ge for research use2 ka809010718pp 18 1 hitrap capto impres q sp compared with hitrap hp q sp delivers similar protein purity and shorter loading time with viscous samples such as e. Cell culture protein a capture step virus inactivation and filtration virus filtration and formulation capto adhere impres polishing step. Cytivahitrap capto sp impres iex columns promo 5 x 5 ml. Capto s impact chromatography medium resin is a strong cation exchanger fig 1. Capto sp impres capto q ft mabselect sure mabselect sure lx twostep process threestep process capto adhere ft capto adhere impres ft or be fig 1. Capto sp impres strong cation and capto q impres strong anion enable highthroughput, highresolution polishing and flexibility in process design. Cv aggregate content at 90% yield % hcp reduction factor protein a ngml start final capto mmc impres 9 0.
Begin with a strong exchanger q, s, sp to enable development work to be performed over a broad ph. Capto phenyl high sub is a hydrophobic interaction chromatography hic medium used in the capture and intermediate stages of protein purification. For viscous buffers and samples the liquid velocity must be optimized and starting with a low flow rate is recommended in order not to exceed pressure limits. Capto s, capto q and capto deae are based on the same. The ability to run at higher flow rates and higher bed heights increases productivity and flexibility in process design. Use of multimodal chromatography media for mab purification. Selecting the option will show capto icon in menu bar. Packing factors were calculated for optimal bed performance where the bed is consolidated at 30 to 60 cmh. Ion exchange columns and media scientific laboratory supplies ltd. Hiscreen capto phenyl impres column characteristics hiscreen columns are made of biocompatible polypropylene that does not interact with biomolecules. Capto sp impres ion exchange chromatography resin with a high binding capacity and small bead size, capto sp impres strong cation exchanger is a good choice for reliable and robust polishing steps in industrial purification.
Highresolution intermediate purification and polishing media based on the wellestablished capto platform with traditional ligands. Flow rate capto sp impres and capto q impres are strong cation and strong anion exchangers, respectively. Capto sp impres and capto q impres are strong cation and strong anion exchangers, respectively. Hiscreen capto sp impres cytiva, 17546815 sigmaaldrich. Hitrap capto sp impres cytiva, 17546855, pack of 5. Packing capto s, capto q, and capto deae in production. Capto sp impres is a strong cation exchange bioprocess medium, designed to meet the demands of modern largescale manufacturers for fast, efficient, and costeffective intermediate and polishing protein purification. Pressureflow curve for capto s compared to q sepharose fast flow. Capto sp impres is a strong cation exchanger for the highthroughput intermediate purification and polishing steps of a wide range of biomolecules.
Efficient aggregate removal at high load of monoclonal antibodies. Please read these instructions carefully before using. Hitrap capto sp impres is a strong cation exchanger prepacked with bioprocess capto impres media for screening and smallscale protein purifications using ion exchange chromatography iexchromatography columns separate molecules within mixtures during gas or liquid chromatography. Separation3 on a capto hires q 550 column separation3 on a resource q 1ml column source 15 resin separation3 on a hitrap q hp 1ml column sepharose high performance resin separation3 on a hitrap capto q impres 1 ml column type of purification manual or system purification system purification format bottles of chromatography resins small. Highthroughput medium for intermediate and polishing protein purification, excellent choice for. Capto q impres is a strong anion exchanger for the highthroughput intermediate purification and polishing steps of a wide range of biomolecules. Capto sp impres and capto q impres are strong cation and strong anion exchangers for the highthroughput intermediate purification and polishing steps of a. Mar 08, 2021 capto sp impres, ml from ge healthcare life sciences. Capto mmc impres fig 1 is a bioprocess chromatography.
Capto s impact ion exchange chromatography resin cytiva. Protein preparation handbook thermo fisher scientific. Pdf purification of antibody fragments for the reduction of. Capto adhere impres can be operated both in flowthrough ft and bindelute be mode. Impact 50 m, and capto impres 40 m iex media are summarized in chapter 3. Capto sp impres and capto q impres are strong cation and strong anion exchangers for the highthroughput intermediate purification and polishing steps of a wide range of biomolecules. Capto s impact is part of a platform of media based on the capto product line. Data file 11002576 ag ion exchange chromatography capto. Typically, an elution from capto adhere impres is performed by a decrease of ph, sometimes combined with change in conductivity. Data file 28988634 af highthroughput process development. Capto s, capto q and capto deae are designed to increase speed, capacity, and throughput in the capture and intermediate purification of biomolecules.
Capto phenyl high sub combines high capacity, high flow rate and low backpressure reduces process cycle times and increases productivity. Selection guide ion exchange chromatography columns and resins. The ligand of capto sp impres is a sulfonate group while the ligand of capto q impres is a quaternary amine group fig 2. Data file 29067018 ab ion exchange chromatography capto. Capto adhere impres multimodal chromatography resin.
Product support capto phenyl impres is a hic resin for highresolution intermediate and polishing steps in a downstream purification process. Polishing of a domain antibody using capto mmc impres. Changing the library location will require relaunching capto. Hiscreen capto l ion exchange chromatography iex hiscreen capto q impres hiscreen capto sp impres hiscreen capto q hiscreen capto s hiscreen capto s impact hiscreen capto deae hiscreen q ff hiscreen sp ff hiscreen deae ff hiscreen q hp hiscreen sp hp multimodal chromatography hiscreen capto core 700 hiscreen capto mmc hiscreen capto adhere. Capto s impact capto sp impres eshmuno cpx fractogel emd so 3m nuvia hrs poros xs mab a, ph 5. By offering high capacity at high flow velocities with low back pressure, process cycle times can be reduced and productivity increased. Static binding capacities of capto sp impres a and capto mmc impres b in the htpd study.
The medium is designed for polishing of monoclonal antibodies mabs and a wide range of other biomolecules. This chromatography resin is part of an expanded highresolution platform based on the highflow agarose capto product line. Data file 29035674 ab multimodal chromatography capto. Preparing the resin to form a slurry can be performed manually. Capto resins are based on highly rigid agarose that allows high flow, which is an important factor for raising productivity in largescale operations. Capto adhere impres is a polishing medium for highly efficient removal of contaminants such as dna, hcp, leached protein a, aggregates, and viruses. Get to know capto from these video tutorials that explain the basics of major features. Capto mmc impres, and capto adhere impres media in largescale chromatography columns. The pressureflow specifications for capto s, capto q, and capto deae in large columns is 700 cmh, with less than 3 bar over a 20 cm bed height, as tested with water at 20c. Capto sp impres is a bioprocess chromatography medium developed for largescale purification of biopharmaceutical proteins. Procedure 29108295 aa multimodal chromatography media mab. To view the contents, you can do a right click control click on capto library and click on show package contents. Data file 11002576 ag ion exchange chromatography capto s.
Hitrap capto s, 1 ml hitrap sp xl, 1 ml hitrap sp ff, 1 ml sample. Hitrap capto sp impres chromatography column is packed with a strong cation exchange modern resin for high resolution, smallscale protein purification. Shop a large selection of protein purification reagents and kits products and learn more about cytivahiscreen capto q impres and capto sp impres. Pdf data file capto sp impres, capto q impres cytiva. Impres capto sp impres capto s impact mab separation cation exchanger anion anion exchanger capto s capto capto deae capto xp predictor screening plates capto. Data file 29035674 ab multimodal chromatography capto mmc. Capto sp impres capto s capto q capto adhere capto q, capto adhere, capto q impres virus inactivation and filtration virus filtration and final formulation capto adhere cell removal cell culture 0 a 280 mau 0 51 10 52 02 53 0 ml 50 100 150 200 250 300 column. Packing capto s, capto q, and capto deae in productionscale. Safety for use and handling of the product in a safe way, please refer to the safety data sheet. Capto mmc impres is a multimodal cation exchange polishing. Capto adhere impres in ft mode resulted in higher yield and lower pool volumes with similar purity table 3. Chromatograms showing the relative resolution in removal of aggregates for capto mmc impres and capto sp impres are shown in figure 3. Capto sp impres has a strong sulfonate cation exchanger coupled to a chemically modified, highflow agarose matrix. The strong ion exchange group of capto sp impres left and capto q.
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